Universal V8 RNA-seq Library Prep Kit for Illumina by AffiGEN

AffiNGS Universal V8 RNA-seq Library Prep Kit for Illumina is meticulously crafted for creating transcriptome libraries on the Illumina platform. It's versatile, fitting RNA library construction from RNA derived via Poly(A)-based mRNA enrichment or rRNA depletion. The kit offers two types of cDNA 2nd Strand synthesis buffers, enabling library construction for both non-stranded and stranded RNA-Seq transcriptome analysis. This kit streamlines the process by combining 2nd Strand cDNA synthesis, end-repair, and dA-Tailing into a single step, eliminating the need for purification and reducing operation time. The optimized reaction system enhances library construction efficiency, accommodates lower Input RNA, and ensures uniform coverage regardless of Input RNA amounts. Customizable libraries of specific sizes can be achieved post size selection with magnetic beads. All kit components undergo stringent quality control and functional testing, ensuring optimal stability and repeatability.

• Optimized for Illumina: Seamless integration
• Versatile RNA library construction
• Dual cDNA 2nd Strand synthesis buffer options
• Streamlined process: No purification needed
• Enhanced library construction performance
• Adaptable to lower input RNA levels
• Consistent coverage across varying input RNA amounts
• Customizable library sizes (magnetic bead selection)
• Thorough quality control and functional assessment